Does it matter which PCIe slot I put my graphics card in?

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Hi, I have R6E mainboard and one ASUS GTX 1080 TI OC graphics card The manual says to put it into the first PCIE slot Unfortunately I

Go! Consumer · Enterprise PRODUCTS Consumer Motherboard AORUS Unlock the latch of the PCIe slot easily when remove the graphics card in the PCIe slot  The different slot types available are an important consideration when buying a graphics card or computer This guide describes the differences between

agent slot online If you ever looked at a PCI to PCIe x16 adapter and wondered what'd happen if you were to stick a modern PCIe GPU in it, Which motherboard slot should you install a new high-end graphics card in? The PCIe ×16 primary slot top near to CPU is the one